PP120 Fluorescent In Situ Hybridization (FISH) Vs Conventional Cytogenetic (CC) For Detecting High-Risk Genetic Mutations In Multiple Myeloma

Introduction The Revised International Staging System (R-ISS) Myeloma Working Group (IMWG) recommends a minimal panel to detect high-risk cytogenetics (del17p, t[4;14], t[14;16]) for patients with multiple myeloma (MM). In the Brazilian Public Health System, use of FISH is currently authorized rare diseases only, not including MM. 2021, National Committee Technology Incorporation, purpose broadening MM patients, requested review be undertaken by Assessment Center University Campinas’ Teaching Hospital. This study presents results meta-analysis comparing vs CC detection above-mentioned aberrations in patients. Methods On 25 June pre-structured search on four databases (Embase, MEDLINE, Cochrane and LILACS) was performed identify studies patients’ bone marrow samples. Study selection, risk bias assessment, data extraction (frequency positive tests) quality evidence assessment were two independent researchers. Conflicts solved agreement meetings third researcher. Meta-analysis using frequency positives obtain Risk Difference (RD), surrogate measure surplus tests between CC. Results From total 1346 rendered entries, 11 selected. Only observational available. These presented an overall high (QUADAS-2). A 781 assessed (653 evaluated 719 CC). showed that, t(4;14) detected 12 percent more samples (RD:0.12 [95% confidence interval (CI):0.06-0.19]). For t(14;16), 0.42 (RD:0.00 [95%CI:-0.01-0.02]). And del17p, 1.6 [95%CI:0.04-0.20]). Conclusions appears effective than t(4;414) del17p aberrations, can useful tool hematology practice. t(14;16) non-superiority, probably due low this aberration.